White Blood Cell Preparation - Step 20
Now is the critical time. The frozen cells and lysosomes will break open mixing their contents. Cysteine, glutathione, thiol groups on proteins, and the cystine we want to measure may react with one another. For that reason, the thawing part of the freeze/thaw cycle should be as short as possible in order to keep the suspension cold and limit it's reactions. Hold the tube in warm water until just thawed and no longer. Do the freeze/thaw cycle a total of three times.